yhdK

168

anti-SigM factor, with YhdL, responds to the free pool of the carrier lipid undecaprenyl-phosphate

Locus: BSU_09500

Molecular weight: 10.50 kDa

Isoelectric point: 8.13

Protein length: 96 aa

Gene length: 291 bp

Function: control of SigM activity

Product: anti-SigM protein

Essential: no

Synonyms: yhdK

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,028,233  1,028,523

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• small colonies on LB PubMed

The protein

Structure

• O07580 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: sigM-yhdL-yhdK

  Description: PubMed

  Regulation

  • induced by glucose PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigM: sigma factor, PubMed, in sigM regulon

  Additional information

  • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed

Biological materials

Mutant

• MGNA-A694 (yhdK::erm), available at the NBRP B. subtilis, Japan
• BP97 (Δ(sigM-yhdL-yhdK)::aphA3), available in Fabian Commichau's lab
• BKE09500 (yhdK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACATTGTGTTCTTTAAAAA,  downstream forward: _UP4_TAAAAAAGACGCCTTTTCAG
• BKK09500 (yhdK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TACATTGTGTTCTTTAAAAA,  downstream forward: _UP4_TAAAAAAGACGCCTTTTCAG

References

Reviews

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Original Publications

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