DivIC

• Description: cell-division initiation protein (septum formation), component of septosome (with DivIB)
  
  

Gene name	divIC
Synonyms
Essential	yes PubMed
Product	cell-division protein
Function	septum formation
Gene expression levels in SubtiExpress: divIC
Interactions involving this protein in SubtInteract: DivIC
MW, pI	14 kDa, 9.968
Gene length, protein length	375 bp, 125 aa
Immediate neighbours	yabQ, yabR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, sporulation proteins, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes, membrane proteins

This gene is a member of the following regulons

SigE regulon, SigM regulon, SigW regulon, SigX regulon

The gene

Basic information

• Locus tag: BSU00620

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: stabilizes FtsL against RasP cleavage PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DivIC-FtsL PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome

• Localization: membrane bound PubMed

Database entries

• BsubCyc: BSU00620

• Structure:

• UniProt: P37471

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • yabM-yabN-yabO-yabP-yabQ-divIC-yabR PubMed
  • yabP-yabQ-divIC-yabR PubMed
  • divIC-yabR PubMed

• Expression browser: divIC PubMed

• Sigma factor:
  • yabM: unknown
  • yabP: SigE PubMed
  • divIC: SigM PubMed, SigX PubMed, SigW PubMed

• Regulation: expressed during sporulation (SigE) PubMed and under stress conditions

• Regulatory mechanism:

• Additional information: there are about 50,000 molecules of DivIC per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Inga Wadenpohl, Marc Bramkamp
DivIC stabilizes FtsL against RasP cleavage.
J Bacteriol: 2010, 192(19);5260-3
[PubMed:20644139] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Richard A Daniel, Marie-Françoise Noirot-Gros, Philippe Noirot, Jeff Errington
Multiple interactions between the transmembrane division proteins of Bacillus subtilis and the role of FtsL instability in divisome assembly.
J Bacteriol: 2006, 188(21);7396-404
[PubMed:16936019] [WorldCat.org] [DOI] (P p)

Scott A Robson, Katharine A Michie, Joel P Mackay, Elizabeth Harry, Glenn F King
The Bacillus subtilis cell division proteins FtsL and DivIC are intrinsically unstable and do not interact with one another in the absence of other septasomal components.
Mol Microbiol: 2002, 44(3);663-74
[PubMed:11994149] [WorldCat.org] [DOI] (P p)

Kei Asai, Hiromu Takamatsu, Megumi Iwano, Takeko Kodama, Kazuhito Watabe, Naotake Ogasawara
The Bacillus subtilis yabQ gene is essential for formation of the spore cortex.
Microbiology (Reading): 2001, 147(Pt 4);919-927
[PubMed:11283287] [WorldCat.org] [DOI] (P p)

V L Katis, R G Wake, E J Harry
Septal localization of the membrane-bound division proteins of Bacillus subtilis DivIB and DivIC is codependent only at high temperatures and requires FtsZ.
J Bacteriol: 2000, 182(12);3607-11
[PubMed:10852898] [WorldCat.org] [DOI] (P p)

J Sievers, J Errington
The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC.
Mol Microbiol: 2000, 36(4);846-55
[PubMed:10844672] [WorldCat.org] [DOI] (P p)

N King, O Dreesen, P Stragier, K Pogliano, R Losick
Septation, dephosphorylation, and the activation of sigmaF during sporulation in Bacillus subtilis.
Genes Dev: 1999, 13(9);1156-67
[PubMed:10323866] [WorldCat.org] [DOI] (P p)

V L Katis, R G Wake
Membrane-bound division proteins DivIB and DivIC of Bacillus subtilis function solely through their external domains in both vegetative and sporulation division.
J Bacteriol: 1999, 181(9);2710-8
[PubMed:10217758] [WorldCat.org] [DOI] (P p)

R A Daniel, E J Harry, V L Katis, R G Wake, J Errington
Characterization of the essential cell division gene ftsL(yIID) of Bacillus subtilis and its role in the assembly of the division apparatus.
Mol Microbiol: 1998, 29(2);593-604
[PubMed:9720875] [WorldCat.org] [DOI] (P p)

V L Katis, E J Harry, R G Wake
The Bacillus subtilis division protein DivIC is a highly abundant membrane-bound protein that localizes to the division site.
Mol Microbiol: 1997, 26(5);1047-55
[PubMed:9426141] [WorldCat.org] [DOI] (P p)

P A Levin, R Losick
Characterization of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation.
J Bacteriol: 1994, 176(5);1451-9
[PubMed:8113187] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed