Difference between revisions of "SppA"
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* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** self-processes its own C-termini {{PubMed|24228759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
− | <pubmed>9987136,12207695 22472423 23980836</pubmed> | + | <pubmed>9987136,12207695 22472423 23980836 24228759 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 09:13, 19 November 2013
- Description: signal peptide peptidase required for efficient processing of pre-proteins
Gene name | sppA |
Synonyms | yteI |
Essential | no |
Product | signal peptide peptidase |
Function | protein secretion |
Gene expression levels in SubtiExpress: sppA | |
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
MW, pI | 36 kDa, 7.314 |
Gene length, protein length | 1005 bp, 335 aa |
Immediate neighbours | yteJ, ytdI |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
protein secretion, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29530
Phenotypes of a mutant
- more sensitive to nisin PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- serine protease that functions to cleave the remnant signal peptides left behind after protein secretion and cleavage by signal peptidases
- Protein family: peptidase S49 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- the protein forms a dome shaped octameric complex PubMed
- Localization:
- cell membrane PubMed
Database entries
- UniProt: O34525
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- self-processes its own C-termini PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Sung-Eun Nam, Mark Paetzel
Structure of signal peptide peptidase A with C-termini bound in the active sites: insights into specificity, self-processing, and regulation.
Biochemistry: 2013, 52(49);8811-22
[PubMed:24228759]
[WorldCat.org]
[DOI]
(I p)
Anthony W Kingston, Xiaojie Liao, John D Helmann
Contributions of the σ(W) , σ(M) and σ(X) regulons to the lantibiotic resistome of Bacillus subtilis.
Mol Microbiol: 2013, 90(3);502-18
[PubMed:23980836]
[WorldCat.org]
[DOI]
(I p)
Sung-Eun Nam, Apollos C Kim, Mark Paetzel
Crystal structure of Bacillus subtilis signal peptide peptidase A.
J Mol Biol: 2012, 419(5);347-58
[PubMed:22472423]
[WorldCat.org]
[DOI]
(I p)
Min Cao, Tao Wang, Rick Ye, John D Helmann
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons.
Mol Microbiol: 2002, 45(5);1267-76
[PubMed:12207695]
[WorldCat.org]
[DOI]
(P p)
X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136]
[WorldCat.org]
[DOI]
(P p)