YdaP

• Description: general stress protein, required for survival of ethanol stress, putative pyruvate oxidase
  
  

Gene name	ydaP
Synonyms
Essential	no
Product	putative pyruvate oxidase
Function	unknown
Gene expression levels in SubtiExpress: ydaP
MW, pI	62 kDa, 5.217
Gene length, protein length	1722 bp, 574 aa
Immediate neighbours	mutT, ydzK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), poorly characterized/ putative enzymes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU04340

Database entries

• BsubCyc: BSU04340

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): AlsS, IlvB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU04340

• Structure:
  • 4KGD (the enzyme from Lactobacillus plantarum, 37% identity, 70% similarity) PubMed

• UniProt: P96591

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ydaP PubMed

• Expression browser: ydaP PubMed

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP457 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Danilo Meyer, Piotr Neumann, Ralf Ficner, Kai Tittmann
Observation of a stable carbene at the active site of a thiamin enzyme.
Nat Chem Biol: 2013, 9(8);488-90
[PubMed:23748673] [WorldCat.org] [DOI] (I p)

Danilo Meyer, Piotr Neumann, Eline Koers, Hanno Sjuts, Stefan Lüdtke, George M Sheldrick, Ralf Ficner, Kai Tittmann
Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin.
Proc Natl Acad Sci U S A: 2012, 109(27);10867-72
[PubMed:22730460] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)