SndA

• Description: sulphur compound N-deacetylase, required for the conversion of S-methyl-cysteine to cysteine
  
  

Gene name	sndA
Synonyms	hipO, ytnL
Essential	no
Product	sulphur compound N-deacetylase
Function	utilization of S-methyl-cysteine
Gene expression levels in SubtiExpress: sndA
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD
MW, pI	45 kDa, 5.545
Gene length, protein length	1248 bp, 416 aa
Immediate neighbours	ytnM, ribR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, AscR regulon, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU29290

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M20 family (according to Swiss-Prot)

• Paralogous protein(s): SndB, SndC, YkuR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34980

• KEGG entry: [3]

• E.C. number:

Additional information

The gene was annotated in KEGG and MetaCyc as hippurate hydrolase EC 3.5.1.32. In Swiss-Prot it is marked as “uncharacterized hydrolase”. No literature/experimental evidence supporting ththeannotation was removed as of November 2009. PubMed

Expression and regulation

• Operon: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM PubMed

• Expression browser: sndA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed
  • induced in the presence of S-methyl-cysteine (AscR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • AscR: transcription activation PubMed

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: