Difference between revisions of "UreA"

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(Original publications)
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36660 ureA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36660 ureA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/UreA UreA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=UreA UreA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate], [http://subtiwiki.uni-goettingen.de/pathways/other_nitrogen_sources/index.html Alternative nitrogen sources]'''

Revision as of 09:32, 12 November 2013

  • Description: urease (gamma subunit)

Gene name ureA
Synonyms
Essential no
Product urease (gamma subunit)
Function utilization of urea as alternative nitrogen source
Gene expression levels in SubtiExpress: ureA
Interactions involving this protein in SubtInteract: UreA
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Ammonium/ glutamate, Alternative nitrogen sources
MW, pI 11 kDa, 4.98
Gene length, protein length 315 bp, 105 aa
Immediate neighbours ureB, ywzE
Sequences Protein DNA DNA_with_flanks
Genetic context
UreA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
UreA expression.png















Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

CodY regulon, GlnR regulon, PucR regulon, SigH regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU36660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Urea + H2O = CO2 + 2 NH3 (according to Swiss-Prot)
  • Protein family: urease gamma subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2FVH (from Mycobacterium tuberculosis, 57% identity, 62% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by nitrogen limitation (GlnR, TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Mark A Farrugia, Lee Macomber, Robert P Hausinger
Biosynthesis of the urease metallocenter.
J Biol Chem: 2013, 288(19);13178-85
[PubMed:23539618] [WorldCat.org] [DOI] (I p)

Stephen W Ragsdale
Nickel-based Enzyme Systems.
J Biol Chem: 2009, 284(28);18571-5
[PubMed:19363030] [WorldCat.org] [DOI] (P p)

Original publications