SdhA

• Description: succinate dehydrogenase (flavoprotein subunit)
  
  

Gene name	sdhA
Synonyms	citF
Essential	no
Product	succinate dehydrogenase (flavoprotein subunit)
Function	TCA cycle
Gene expression levels in SubtiExpress: sdhA
Interactions involving this protein in SubtInteract: SdhA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	65 kDa, 5.714
Gene length, protein length	1758 bp, 586 aa
Immediate neighbours	sdhB, sdhC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

FsrA regulon

The gene

Basic information

• Locus tag: BSU28440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Succinate + acceptor = fumarate + reduced acceptor (according to Swiss-Prot)

• Protein family: FRD/SDH subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated PubMed, PubMed

• Cofactor(s): Fe

• Effectors of protein activity:
  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
  • Activated by Cytochrome b558 PubMed

• Interactions:
  • SdhA-SdhB (as part of the SdhC-SdhB-SdhA complex)
  • FloT-SdhA PubMed
  • part of a respiratory supercomplex PubMed

• Localization:
  • attached to the membrane PubMed

Database entries

• Structure: 1NEK (E. coli)

• UniProt: P08065

• KEGG entry: [3]

• E.C. number: 1.3.99.1

Additional information

• This enzyme is a membrane-bound trimer PubMed PubMed
  • One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
  • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
• extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia

Expression and regulation

• Operon: sdhC-sdhA-sdhB PubMed

• Expression browser: sdhA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • constitutive
  • part of the iron sparing response (FsrA) PubMed

• Regulatory mechanism:
  • FsrA: translation inhibition PubMed

• Additional information:

Biological materials

• Mutant: GP743 (sdhCA, cat), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

References

Reviews

Lars Hederstedt
Succinate:quinone oxidoreductase in the bacteria Paracoccus denitrificans and Bacillus subtilis.
Biochim Biophys Acta: 2002, 1553(1-2);74-83
[PubMed:11803018] [WorldCat.org] [DOI] (P p)