GerW

• Description: germination protein, essential for germination in response to L-alanine
  
  

Gene name	gerW
Synonyms	ytfJ
Essential	no
Product	germination protein
Function	germination in response to L-alanine
Gene expression levels in SubtiExpress: gerW
MW, pI	16 kDa, 4.988
Gene length, protein length	453 bp, 151 aa
Immediate neighbours	tpx, ytfI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU29500

Phenotypes of a mutant

• no germination in response to l-alanine PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • in the dormant spore PubMed

Database entries

• Structure:

• UniProt: O34806

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ytfI-gerW PubMed

• Expression browser: gerW PubMed

• Sigma factor: SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jose Cruz-Mora, Abigail Pérez-Valdespino, Srishti Gupta, Nilumi Withange, Ritsuko Kuwana, Hiromu Takamatsu, Graham Christie, Peter Setlow
The GerW protein is not involved in the germination of spores of Bacillus species.
PLoS One: 2015, 10(3);e0119125
[PubMed:25790435] [WorldCat.org] [DOI] (I e)

Ritsuko Kuwana, Hiromu Takamatsu
The GerW protein is essential for L-alanine-stimulated germination of Bacillus subtilis spores.
J Biochem: 2013, 154(5);409-17
[PubMed:23921501] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)