Difference between revisions of "RnhB"

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* '''Description:''' [[RNase]] HII, endoribonuclease <br/><br/>
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* '''Description:''' [[RNase]] HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during [[DNA replication]] <br/><br/>
  
 
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{| align="right" border="1" cellpadding="2"  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* increased mutation rate {{PubMed|23882084}}
 
* a ''[[rnhB]] [[rnhC]]'' double mutant grows poorly {{PubMed|23882084}}
 
* a ''[[rnhB]] [[rnhC]]'' double mutant grows poorly {{PubMed|23882084}}
 +
* a ''[[rnhB]] [[mutS]]-[[mutL]]'' mutant is not viable {{PubMed|23882084}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)  
 +
** removal of single rNMPs incorporated into DNA by DNA polymerase during [[DNA replication]]  {{PubMed|23882084}}
  
 
* '''Protein family:''' [[RNase]] HII family (according to Swiss-Prot)
 
* '''Protein family:''' [[RNase]] HII family (according to Swiss-Prot)

Revision as of 15:57, 25 July 2013

  • Description: RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication

Gene name rnhB
Synonyms rnh
Essential no
Product Mn2+-dependent RNase HII
Function endonucleolytic cleavage of RNA in
RNA-DNA hybrid molecules
Gene expression levels in SubtiExpress: rnhB
MW, pI 28 kDa, 5.518
Gene length, protein length 765 bp, 255 aa
Immediate neighbours rbgA, ylqG
Sequences Protein DNA DNA_with_flanks
Genetic context
RnhB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RnhB expression.png















Categories containing this gene/protein

DNA replication, Rnases

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16060

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
    • removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed
  • Protein family: RNase HII family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number: 3.1.26.4.

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084] [WorldCat.org] [DOI] (I p)

Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985] [WorldCat.org] [DOI] (I p)

Mitsuru Haruki, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya
Cleavage of a DNA-RNA-DNA/DNA chimeric substrate containing a single ribonucleotide at the DNA-RNA junction with prokaryotic RNases HII.
FEBS Lett: 2002, 531(2);204-8
[PubMed:12417313] [WorldCat.org] [DOI] (P p)

M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689] [WorldCat.org] [DOI] (P p)

N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800] [WorldCat.org] [DOI] (P p)