Difference between revisions of "SrfAA"

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(Original publications)
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=== Additional information===
 
=== Additional information===
 
* A mutation was found in this gene after evolution under relaxed selection for sporulation  {{PubMed|21821766}}
 
* A mutation was found in this gene after evolution under relaxed selection for sporulation  {{PubMed|21821766}}
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:''' phosphorylation on Ser-1006 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
 
* '''Modification:''' phosphorylation on Ser-1006 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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<pubmed>20735481 </pubmed>
 
<pubmed>20735481 </pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>8288534,8387999,1938922,16166527,17227471,8825779, 8830686,18763711 17218307, 17190806 1715856 19605685 19826092 12642660 20148996 16091051 20797616 20525796 21821766 23012477 22511326,20817675,15066026</pubmed>
+
<pubmed>8288534,8387999,1938922,16166527,17227471,8825779, 8830686,18763711 17218307, 17190806 1715856 19605685 19826092 12642660 20148996 16091051 20797616 20525796 21821766 23012477 22511326,20817675,15066026 24425772 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:18, 17 January 2014

  • Description: surfactin synthetase / competence

Gene name srfAA
Synonyms comL
Essential no
Product surfactin synthetase / competence
Function antibiotic synthesis
Gene expression levels in SubtiExpress: srfAA
MW, pI 401 kDa, 4.871
Gene length, protein length 10764 bp, 3588 aa
Immediate neighbours hxlR, srfAB
Sequences Protein DNA DNA_with_flanks
Genetic context
SrfAA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SrfAA expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, Spx regulon

The gene

Basic information

  • Locus tag: BSU03480

Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed
  • reduced swarming of srfAA mutant of B. subtilis NCIB3610 PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylation on Ser-1006 PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
    • repressed during hydrogen peroxide stress (PerR) PubMed
    • expressed at high cell density (ComA) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications