Csn

• Description: chitosanase
  
  

Gene name	csn
Synonyms
Essential	no
Product	chitosanase
Function	chitin degradation
Gene expression levels in SubtiExpress: csn
MW, pI	31 kDa, 8.892
Gene length, protein length	831 bp, 277 aa
Immediate neighbours	yraM, yraL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon

The gene

Basic information

• Locus tag: BSU26890

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 46 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide), major constituent of the secretome PubMed

Database entries

• Structure:

• UniProt: O07921

• KEGG entry: [2]

• E.C. number: 3.2.1.132

Expression and regulation

• Operon:

• Expression browser: csn PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Chulhong Oh, Mahanama De Zoysa, Do-Hyung Kang, Youngdeuk Lee, Ilson Whang, Chamilani Nikapitiya, Soo-Jin Heo, Kon-Tak Yoon, Abu Affan, Jehee Lee
Isolation, purification, and enzymatic characterization of extracellular chitosanase from marine bacterium Bacillus subtilis CH2.
J Microbiol Biotechnol: 2011, 21(10);1021-5
[PubMed:22031025] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Anne Colomer-Pallas, Yannick Pereira, Marie-Françoise Petit-Glatron, Régis Chambert
Calcium triggers the refolding of Bacillus subtilis chitosanase.
Biochem J: 2003, 369(Pt 3);731-8
[PubMed:12401130] [WorldCat.org] [DOI] (P p)

Luis A Rivas, Vı Ctor Parro, Mercedes Moreno-Paz, Rafael P Mellado
The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a streptomyces enzyme.
Microbiology (Reading): 2000, 146 ( Pt 11);2929-2936
[PubMed:11065371] [WorldCat.org] [DOI] (P p)