Difference between revisions of "RacE"

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(Basic information/ Evolution)
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=racE_2903217_2904035_-1 racE] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=racE_2903217_2904035_-1 racE] {{PubMed|22383849}}
  
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=References=
 
=References=
  
<pubmed>15347750,16271894 , 19309142, 19227983 </pubmed>
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<pubmed>15347750,16271894 , 19309142, 19227983 23929705 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:13, 10 August 2013

  • Description: glutamate racemase

Gene name racE
Synonyms glr
Essential yes PubMed
Product glutamate racemase
Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: racE
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 29 kDa, 4.858
Gene length, protein length 816 bp, 272 aa
Immediate neighbours gerM, ysmB
Sequences Protein DNA DNA_with_flanks
Genetic context
RacE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RacE expression.png















Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU28390

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-glutamate = D-glutamate (according to Swiss-Prot)
  • Protein family: aspartate/glutamate racemases family (according to Swiss-Prot)
  • Paralogous protein(s): YrpC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZUW (with D-glutamate)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Katie L Whalen, Anthony C Chau, M Ashley Spies
In silico optimization of a fragment-based hit yields biologically active, high-efficiency inhibitors for glutamate racemase.
ChemMedChem: 2013, 8(10);1681-9
[PubMed:23929705] [WorldCat.org] [DOI] (I p)

M Ashley Spies, Joseph G Reese, Dylan Dodd, Katherine L Pankow, Steven R Blanke, Jerome Baudry
Determinants of catalytic power and ligand binding in glutamate racemase.
J Am Chem Soc: 2009, 131(14);5274-84
[PubMed:19309142] [WorldCat.org] [DOI] (I p)

Eduard Puig, Edgar Mixcoha, Mireia Garcia-Viloca, Angels González-Lafont, José M Lluch
How the substrate D-glutamate drives the catalytic action of Bacillus subtilis glutamate racemase.
J Am Chem Soc: 2009, 131(10);3509-21
[PubMed:19227983] [WorldCat.org] [DOI] (I p)

Sergey N Ruzheinikov, Makie A Taal, Svetlana E Sedelnikova, Patrick J Baker, David W Rice
Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery.
Structure: 2005, 13(11);1707-13
[PubMed:16271894] [WorldCat.org] [DOI] (P p)

Keitarou Kimura, Lam-Son Phan Tran, Yoshifumi Itoh
Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis.
Microbiology (Reading): 2004, 150(Pt 9);2911-2920
[PubMed:15347750] [WorldCat.org] [DOI] (P p)