Difference between revisions of "LigD"
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| − | * '''Description:''' | + | * '''Description:''' DNA repair polymerase/ ligase in non-homologous end joining DNA repair <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA repair polymerase/ ligase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || non-homologous end joining DNA repair | |style="background:#ABCDEF;" align="center"|'''Function''' || non-homologous end joining DNA repair | ||
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13400 ligD] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13400 ligD] | ||
| + | |- | ||
| + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/LigD LigD] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 6.646 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 6.646 | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | + | * sensitivity to ionizing radiation in the stationary phase {{PubMed|12215643}} | |
| + | * sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat {{PubMed|16497325,17293412}} | ||
=== Database entries === | === Database entries === | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** [[YkoV]]-[[LigD]] {{PubMed|23691176}} | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| Line 141: | Line 145: | ||
<pubmed> 17938628 </pubmed> | <pubmed> 17938628 </pubmed> | ||
== Original publications == | == Original publications == | ||
| − | <pubmed>16497325, 23691176, 12215643 11075926 11566200 </pubmed> | + | <pubmed>16497325, 23691176, 12215643 11075926 11566200 17293412 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:33, 23 May 2013
- Description: DNA repair polymerase/ ligase in non-homologous end joining DNA repair
| Gene name | ligD |
| Synonyms | ykoU |
| Essential | no |
| Product | DNA repair polymerase/ ligase |
| Function | non-homologous end joining DNA repair |
| Gene expression levels in SubtiExpress: ligD | |
| Interactions involving this protein in SubtInteract: LigD | |
| MW, pI | 70 kDa, 6.646 |
| Gene length, protein length | 1833 bp, 611 aa |
| Immediate neighbours | ykoT, ykoV |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DNA repair/ recombination, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13400
Phenotypes of a mutant
- sensitivity to ionizing radiation in the stationary phase PubMed
- sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: C-terminal DNA ligase catalytic domain linked to an N-terminal polymerase domain PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34398
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- expressed during sporulation in the forespore (SigG, SpoVT) PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628]
[WorldCat.org]
[DOI]
(I p)
Original publications
Miguel de Vega
The minimal Bacillus subtilis nonhomologous end joining repair machinery.
PLoS One: 2013, 8(5);e64232
[PubMed:23691176]
[WorldCat.org]
[DOI]
(I e)
Ralf Moeller, Erko Stackebrandt, Günther Reitz, Thomas Berger, Petra Rettberg, Aidan J Doherty, Gerda Horneck, Wayne L Nicholson
Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore resistance to extreme dryness, mono- and polychromatic UV, and ionizing radiation.
J Bacteriol: 2007, 189(8);3306-11
[PubMed:17293412]
[WorldCat.org]
[DOI]
(P p)
Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325]
[WorldCat.org]
[DOI]
(P p)
Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny A Jeggo, Stephen P Jackson, Aidan J Doherty
Identification of a DNA nonhomologous end-joining complex in bacteria.
Science: 2002, 297(5587);1686-9
[PubMed:12215643]
[WorldCat.org]
[DOI]
(I p)
G R Weller, A J Doherty
A family of DNA repair ligases in bacteria?
FEBS Lett: 2001, 505(2);340-2
[PubMed:11566200]
[WorldCat.org]
[DOI]
(P p)
E V Koonin, Y I Wolf, A S Kondrashov, L Aravind
Bacterial homologs of the small subunit of eukaryotic DNA primase.
J Mol Microbiol Biotechnol: 2000, 2(4);509-12
[PubMed:11075926]
[WorldCat.org]
(P p)
