Difference between revisions of "LigD"

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m (Jstuelk moved page YkoU to LigD: 23691176)
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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ykoU''
+
|''ligD''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ykoU''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"|'''Function''' || non-homologous end joining DNA repair  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || non-homologous end joining DNA repair  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13400 ykoU]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13400 ligD]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 6.646   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 70 kDa, 6.646   
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[ykoV]]-[[ykoU]]'' {{PubMed|16497325}}
+
* '''Operon:''' ''[[ykoV]]-[[ligD]]'' {{PubMed|16497325}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykoU_1404518_1406353_-1 ykoU] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykoU_1404518_1406353_-1 ykoU] {{PubMed|22383849}}

Revision as of 11:04, 23 May 2013

  • Description: confers dry-heat resistance to dormant spores PubMed, similar to ATP-dependent DNA ligase

Gene name ligD
Synonyms ykoU
Essential no
Product unknown
Function non-homologous end joining DNA repair
Gene expression levels in SubtiExpress: ligD
MW, pI 70 kDa, 6.646
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours ykoT, ykoV
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoU expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon


The gene

Basic information

  • Locus tag: BSU13400

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: C-terminal DNA ligase catalytic domain linked to an N-terminal polymerase domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Stewart Shuman, Michael S Glickman
Bacterial DNA repair by non-homologous end joining.
Nat Rev Microbiol: 2007, 5(11);852-61
[PubMed:17938628] [WorldCat.org] [DOI] (I p)

Original publications

Miguel de Vega
The minimal Bacillus subtilis nonhomologous end joining repair machinery.
PLoS One: 2013, 8(5);e64232
[PubMed:23691176] [WorldCat.org] [DOI] (I e)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Geoffrey R Weller, Boris Kysela, Rajat Roy, Louise M Tonkin, Elizabeth Scanlan, Marina Della, Susanne Krogh Devine, Jonathan P Day, Adam Wilkinson, Fabrizio d'Adda di Fagagna, Kevin M Devine, Richard P Bowater, Penny A Jeggo, Stephen P Jackson, Aidan J Doherty
Identification of a DNA nonhomologous end-joining complex in bacteria.
Science: 2002, 297(5587);1686-9
[PubMed:12215643] [WorldCat.org] [DOI] (I p)

G R Weller, A J Doherty
A family of DNA repair ligases in bacteria?
FEBS Lett: 2001, 505(2);340-2
[PubMed:11566200] [WorldCat.org] [DOI] (P p)

E V Koonin, Y I Wolf, A S Kondrashov, L Aravind
Bacterial homologs of the small subunit of eukaryotic DNA primase.
J Mol Microbiol Biotechnol: 2000, 2(4);509-12
[PubMed:11075926] [WorldCat.org] (P p)