Difference between revisions of "DeaD"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39110 deaD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39110 deaD]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DeaD DeaD]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DeaD DeaD]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 7.706   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 7.706   

Revision as of 09:42, 12 November 2013

Gene name deaD
Synonyms yxiN
Essential no
Product DEAD-box RNA helicase
Function RNA helicase
Gene expression levels in SubtiExpress: deaD
Interactions involving this protein in SubtInteract: DeaD
MW, pI 53 kDa, 7.706
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours yxiO, yxiM
Sequences Protein DNA DNA_with_flanks
Genetic context
DeaD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DeaD expression.png















Categories containing this gene/protein

DEAD-box RNA helicases, translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU39110

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Helicase domain 1: AS 1-203
    • Linker region: AS 204-212
    • Helicase domain 2: AS 207-368
    • RNA-binding domain: AS 404-479
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2G0C (RNA-binding domain, AA 404-479), 2HJV (second domain, AA 207-368); 3MOJ (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression declines in the stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1052 (tet), available in the Stülke lab
  • Expression vector:
    • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1065 (spc), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

Your additional remarks

References