Tgl

• Description: outer spore coat protein, spore associated transglutaminase
  
  

Gene name	tgl
Synonyms	yuxF, yugV
Essential	no
Product	spore associated transglutaminase
Function	introduction of crosslinks in the spore coat protein GerQ
Gene expression levels in SubtiExpress: tgl
MW, pI	28 kDa, 6.687
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	mcpB, yuzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon, SigK regulon

The gene

Basic information

• Locus tag: BSU31270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH₃ (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed

• Protein family: bacillus TGase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer spore coat, localization depends on CotE PubMed

Database entries

• Structure:

• UniProt: P40746

• KEGG entry: [3]

• E.C. number: 2.3.2.13

Expression and regulation

• Operon: tgl PubMed

• Expression browser: tgl PubMed

• Sigma factor: SigK PubMed, SigE

• Regulation:
  • expressed late during sporulation in the mother cell (SigE, SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S122 ( tgl::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage