Difference between revisions of "ThiI"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of 4-thiouridine in tRNA
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of 4-thiouridine in tRNA
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/ThiI ThiI]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ThiI ThiI]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 9.663   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 9.663   

Revision as of 14:19, 18 November 2013

  • Description: sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA

Gene name thiI
Synonyms ytbJ
Essential no
Product sulfuryl transferase
Function biosynthesis of 4-thiouridine in tRNA
Interactions involving this protein in SubtInteract: ThiI
MW, pI 40 kDa, 9.663
Gene length, protein length 1074 bp, 358 aa
Immediate neighbours sspA, nifZ
Sequences Protein DNA DNA_with_flanks
Genetic context
YtbJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YtbJ expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29580

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: receives a sulfuryl group from NifZ and transfers it to uridine at position 8 of tRNA PubMed
  • Protein family: thiI family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787] [WorldCat.org] [DOI] (I p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)