Difference between revisions of "Gpr"

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* '''Description:''' spore protease (degradation of SASPs) <br/><br/>
+
* '''Description:''' [[germination]] protease (degradation of SASPs) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || spore protease (degradation of SASPs)
+
|style="background:#ABCDEF;" align="center"| '''Product''' || [[germination]] protease (degradation of SASPs)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || germination
+
|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of SASPs
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25540 gpr]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25540 gpr]
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<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[proteolysis]]}},
 
{{SubtiWiki category|[[germination]]}}
 
{{SubtiWiki category|[[germination]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
{{SubtiWiki regulon|[[SigF regulon]]}},
 
{{SubtiWiki regulon|[[SigF regulon]]}},
{{SubtiWiki regulon|[[SigG regulon]]}}
+
{{SubtiWiki regulon|[[SigG regulon]]}},
 +
{{SubtiWiki regulon|[[SpoVT regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Additional information===
 
=== Additional information===
 
 
  
  
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' degradation of small, acid-soluble spore proteins (SASPs)
+
* '''Catalyzed reaction/ biological activity:''' degradation of small, acid-soluble spore proteins (SASPs) {{PubMed|8478323}}
  
 
* '''Protein family:''' peptidase A25 family (according to Swiss-Prot)
 
* '''Protein family:''' peptidase A25 family (according to Swiss-Prot)
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* '''Sigma factor:''' [[SigF]] {{PubMed|16497325,1840582}}, [[SigG]] {{PubMed|1840582}}
 
* '''Sigma factor:''' [[SigF]] {{PubMed|16497325,1840582}}, [[SigG]] {{PubMed|1840582}}
  
* '''Regulation:''' expressed during sporulation in the forespore ([[SigF]], [[SigG]]) {{PubMed|16497325,1840582}}
+
* '''Regulation:''' expressed during sporulation in the forespore ([[SigF]], [[SigG]], [[SpoVT]]) {{PubMed|16497325,1840582}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[SpoVT]]: transcription repression {{PubMed|16497325}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323, </pubmed>
+
<pubmed>16199582,9045848,8188581,8071242,1732215,16497325,7961468,8071212,9748439,1840582,7608092,8478323, 23927687 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:50, 12 August 2013

  • Description: germination protease (degradation of SASPs)

Gene name gpr
Synonyms
Essential no
Product germination protease (degradation of SASPs)
Function degradation of SASPs
Gene expression levels in SubtiExpress: gpr
MW, pI 40 kDa, 5.199
Gene length, protein length 1104 bp, 368 aa
Immediate neighbours spoIIP, rpsT
Sequences Protein DNA DNA_with_flanks
Genetic context
Gpr context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Gpr expression.png















Categories containing this gene/protein

proteolysis, germination

This gene is a member of the following regulons

SigF regulon, SigG regulon, SpoVT regulon

The gene

Basic information

  • Locus tag: BSU25540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degradation of small, acid-soluble spore proteins (SASPs) PubMed
  • Protein family: peptidase A25 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Bjorn A Traag, Antonia Pugliese, Barbara Setlow, Peter Setlow, Richard Losick
A conserved ClpP-like protease involved in spore outgrowth in Bacillus subtilis.
Mol Microbiol: 2013, 90(1);160-6
[PubMed:23927687] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Thomas M Carroll, Peter Setlow
Site-directed mutagenesis and structural studies suggest that the germination protease, GPR, in spores of Bacillus species is an atypical aspartic acid protease.
J Bacteriol: 2005, 187(20);7119-25
[PubMed:16199582] [WorldCat.org] [DOI] (P p)

C Nessi, M J Jedrzejas, P Setlow
Structure and mechanism of action of the protease that degrades small, acid-soluble spore proteins during germination of spores of Bacillus species.
J Bacteriol: 1998, 180(19);5077-84
[PubMed:9748439] [WorldCat.org] [DOI] (P p)

L B Pedersen, C Nessi, P Setlow
Most of the propeptide is dispensable for stability and autoprocessing of the zymogen of the germination protease of spores of Bacillus species.
J Bacteriol: 1997, 179(5);1824-7
[PubMed:9045848] [WorldCat.org] [DOI] (P p)

B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Autoprocessing of the protease that degrades small, acid-soluble proteins of spores of Bacillus species is triggered by low pH, dehydration, and dipicolinic acid.
J Bacteriol: 1994, 176(22);7032-7
[PubMed:7961468] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
The zymogen of the protease that degrades small, acid-soluble proteins of spores of Bacillus species can rapidly autoprocess to the active enzyme in vitro.
J Bacteriol: 1994, 176(17);5571-3
[PubMed:8071242] [WorldCat.org] [DOI] (P p)

Y Carrillo-Martinez, P Setlow
Properties of Bacillus subtilis small, acid-soluble spore proteins with changes in the sequence recognized by their specific protease.
J Bacteriol: 1994, 176(17);5357-63
[PubMed:8071212] [WorldCat.org] [DOI] (P p)

B Illades-Aguiar, P Setlow
Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species.
J Bacteriol: 1994, 176(10);2788-95
[PubMed:8188581] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, P Setlow
Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores.
J Bacteriol: 1993, 175(9);2568-77
[PubMed:8478323] [WorldCat.org] [DOI] (P p)

J L Sanchez-Salas, M L Santiago-Lara, B Setlow, M D Sussman, P Setlow
Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination.
J Bacteriol: 1992, 174(3);807-14
[PubMed:1732215] [WorldCat.org] [DOI] (P p)

M D Sussman, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.
J Bacteriol: 1991, 173(1);291-300
[PubMed:1840582] [WorldCat.org] [DOI] (P p)