Difference between revisions of "DnaG"

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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1D0Q 1D0Q] (zinc binding domain, Geobacillus stearothermophilus)[http://www.rcsb.org/pdb/explore.do?structureId=1Z8S 1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
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* '''Structure:'''
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** [http://www.rcsb.org/pdb/explore.do?structureId=1D0Q 1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
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** [http://www.rcsb.org/pdb/explore.do?structureId=1Z8S 1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
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** [http://www.rcsb.org/pdb/explore.do?structureId=4M4W 4M4W] (the [[DnaC]]-[[DnaI]]-[[DnaG]] complex) {{PubMed|24048025}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P05096 P05096]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P05096 P05096]
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==Original publications==
 
==Original publications==
<pubmed>3919021, 20122408,14651647, 16479537, 19415803 17350574 23563155 23268446,21419346</pubmed>
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<pubmed>3919021, 20122408,14651647, 16479537, 19415803 17350574 23563155 23268446,21419346 24048025</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:32, 23 September 2013

  • Description: DNA primase, part of the replisome

Gene name dnaG
Synonyms dnaE
Essential yes PubMed
Product DNA primase
Function DNA replication
Gene expression levels in SubtiExpress: dnaG
Interactions involving this protein in SubtInteract: DnaG
MW, pI 68 kDa, 6.706
Gene length, protein length 1809 bp, 603 aa
Immediate neighbours sigA, antE
Sequences Protein DNA DNA_with_flanks
Genetic context
DnaG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaG expression.png















Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU25210

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • required for bacteriophage SPP1 replication PubMed
  • Protein family: DNA primase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • the enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´ PubMed
    • DnaG primase activity is stimulates by DnaC PubMed

Database entries

  • Structure:
    • 1D0Q (zinc binding domain, Geobacillus stearothermophilus)
    • 1Z8S (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
    • 4M4W (the DnaC-DnaI-DnaG complex) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´
  • Regulatory mechanism:
  • Additional information:
    • enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´ PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications