Difference between revisions of "SucD"

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(Biological materials)
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* '''Mutant:''' GP720 (spc), available in [[Stülke]] lab  
 
* '''Mutant:''' GP720 (spc), available in [[Stülke]] lab  
 
** 1A1008 ( ''sucD''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1008&Search=1A1008 BGSC]
 
** 1A1008 ( ''sucD''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1008&Search=1A1008 BGSC]
 +
** GP791 (''sucCD''::''tet''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:05, 13 August 2013

  • Description: succinyl-CoA synthetase (alpha subunit)

Gene name sucD
Synonyms
Essential no
Product succinyl-CoA synthetase (alpha subunit)
Function TCA cycle
Gene expression levels in SubtiExpress: sucD
Interactions involving this protein in SubtInteract: SucD
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 31 kDa, 5.587
Gene length, protein length 900 bp, 300 aa
Immediate neighbours sucC, dprA
Sequences Protein DNA DNA_with_flanks
Genetic context
SucD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SucD expression.png




























Categories containing this gene/protein

ATP synthesis, carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU16100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
  • Protein family: succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten FEBS Letters
  • Domains:
  • Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1JKJ (E. coli)
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of succinyl-CoA synthetase can be found at Proteopedia

Expression and regulation

  • Sigma factor:
  • Regulation: repressed by glucose (2.4-fold) (CcpA) PubMed
  • Regulatory mechanism: CcpA: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion: GP1435 (spc, based on pGP1870), available in the Stülke lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1425 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References