Difference between revisions of "MgtE"

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* '''Description:''' magnesium transport via permease (no H+) <br/><br/>
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* '''Description:''' primary magnesium transporter <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || magnesium transport via permease (no H+)
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|style="background:#ABCDEF;" align="center"| '''Product''' || primary magnesium transporter
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || magnesium uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || magnesium uptake
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<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
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<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* a ''[[mgtE]]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg<sup>2+</sup>, moreover the mutant grows well on minimal medim in the presence of citrate {{PubMed|24415722}}
 +
*  a ''[[mgtE]] [[yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg<sup>2+</sup>, moreover the mutant grows well on minimal medim in the presence of citrate {{PubMed|24415722}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgtE_1396013_1397368_1 mgtE] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgtE_1396013_1397368_1 mgtE] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:''' induced at low magnesium concentration ([[M-box]]) {{PubMed|17803910}}
 
* '''Regulation:''' induced at low magnesium concentration ([[M-box]]) {{PubMed|17803910}}
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=References=
 
=References=
  
<pubmed>17803910,15096624, 21315082, 22970223 </pubmed>
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<pubmed>17803910,15096624, 21315082, 22970223 24415722</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:10, 16 January 2014

  • Description: primary magnesium transporter

Gene name mgtE
Synonyms ykoK
Essential no
Product primary magnesium transporter
Function magnesium uptake
Gene expression levels in SubtiExpress: mgtE
MW, pI 50 kDa, 4.609
Gene length, protein length 1353 bp, 451 aa
Immediate neighbours ykzO, tnrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgtE expression.png















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins

This gene is a member of the following regulons

M-box

The gene

Basic information

  • Locus tag: BSU13300

Phenotypes of a mutant

  • a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
  • a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: SLC41A transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2QBZ (the mgtE riboswitch)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: induced at low magnesium concentration (M-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Catherine A Wakeman, Jonathan R Goodson, Vineetha M Zacharia, Wade C Winkler
Assessment of the requirements for magnesium transporters in Bacillus subtilis.
J Bacteriol: 2014, 196(6);1206-14
[PubMed:24415722] [WorldCat.org] [DOI] (I p)

Jaya Sahni, Yumei Song, Andrew M Scharenberg
The B. subtilis MgtE magnesium transporter can functionally compensate TRPM7-deficiency in vertebrate B-cells.
PLoS One: 2012, 7(9);e44452
[PubMed:22970223] [WorldCat.org] [DOI] (I p)

Arati Ramesh, Catherine A Wakeman, Wade C Winkler
Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.
J Mol Biol: 2011, 407(4);556-70
[PubMed:21315082] [WorldCat.org] [DOI] (I p)

Charles E Dann, Catherine A Wakeman, Cecelia L Sieling, Stephanie C Baker, Irnov Irnov, Wade C Winkler
Structure and mechanism of a metal-sensing regulatory RNA.
Cell: 2007, 130(5);878-92
[PubMed:17803910] [WorldCat.org] [DOI] (P p)

Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624] [WorldCat.org] [DOI] (P p)