Difference between revisions of "ChaA"

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{{SubtiWiki category|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},
 
{{SubtiWiki category|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},
 
{{SubtiWiki category|[[sporulation proteins]]}},
 
{{SubtiWiki category|[[sporulation proteins]]}},
{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}}
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{{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
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{{SubtiWiki category|[[membrane proteins]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** forms a homotrimer {{PubMed|23798403}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 +
** cell membrane {{PubMed|23798403}}
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
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* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4KJS 4KJS] {{PubMed|23798403}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34840 O34840]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34840 O34840]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfkE_865205_866260_1 chaA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfkE_865205_866260_1 chaA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigG]], [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19543710 PubMed]
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* '''[[Sigma factor]]:''' [[SigG]], [[SigB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19543710 PubMed]
  
 
* '''Regulation:''' expressed in the forespore ([[SigG]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19543710 PubMed]
 
* '''Regulation:''' expressed in the forespore ([[SigG]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/19543710 PubMed]
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=References=
 
=References=
<pubmed>19543710 </pubmed>
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<pubmed>19543710 23798403 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:42, 1 July 2013

  • Description: H+/Ca2+ exchanger

Gene name chaA
Synonyms yfkE
Essential no
Product calcium transport in/out via proton antiporter
Function calcium uptake/ export
Gene expression levels in SubtiExpress: chaA
MW, pI 37 kDa, 5.206
Gene length, protein length 1053 bp, 351 aa
Immediate neighbours yfkF, yfkD
Sequences Protein DNA DNA_with_flanks
Genetic context
YfkE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ChaA expression.png















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), sporulation proteins, general stress proteins (controlled by SigB), membrane proteins

This gene is a member of the following regulons

SigB regulon, SigG regulon

The gene

Basic information

  • Locus tag: BSU07920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mousheng Wu, Shuilong Tong, Sandro Waltersperger, Kay Diederichs, Meitian Wang, Lei Zheng
Crystal structure of Ca2+/H+ antiporter protein YfkE reveals the mechanisms of Ca2+ efflux and its pH regulation.
Proc Natl Acad Sci U S A: 2013, 110(28);11367-72
[PubMed:23798403] [WorldCat.org] [DOI] (I p)

Makoto Fujisawa, Yuko Wada, Takahiro Tsuchiya, Masahiro Ito
Characterization of Bacillus subtilis YfkE (ChaA): a calcium-specific Ca2+/H+ antiporter of the CaCA family.
Arch Microbiol: 2009, 191(8);649-57
[PubMed:19543710] [WorldCat.org] [DOI] (I p)