Difference between revisions of "BACTH"
(New page: '''Bacterial Adenylate Cyclase-based two hybrid system''' Allows the detection of in vivo protein-protein interactions by restoring the activity of the ''Bordetella pertussis'' adenylate ...) |
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Active adenylate cyclase results in the expression of the ''lacZ'' gene which is detected by blue colonies on plates containing X-Gal. | Active adenylate cyclase results in the expression of the ''lacZ'' gene which is detected by blue colonies on plates containing X-Gal. | ||
| − | see a scheme of the principle: [ | + | see a scheme of the principle: [http://www.euromedex.com/shop/popup_image.php?pID=208357&osCsid=a63d2e53d8afa86ab50e58493b07b400 picture] |
The system consists of four plasmids and one E. coli strain (BTH101) | The system consists of four plasmids and one E. coli strain (BTH101) | ||
Revision as of 12:08, 15 January 2009
Bacterial Adenylate Cyclase-based two hybrid system
Allows the detection of in vivo protein-protein interactions by restoring the activity of the Bordetella pertussis adenylate cyclase in E. coli reporter strains.
Active adenylate cyclase results in the expression of the lacZ gene which is detected by blue colonies on plates containing X-Gal.
see a scheme of the principle: picture
The system consists of four plasmids and one E. coli strain (BTH101)
- pKT25: C-terminal fusion of the target protein 1 to the T25 fragment of adenylate cyclase
- pKT25-N: N-terminal fusion of the target protein 1 to the T25 fragment of adenylate cyclase
- pUT18: N-terminal fusion of the target protein 1 to the T18 fragment of adenylate cyclase
- pUT18C: C-terminal fusion of the target protein 1 to the T18 fragment of adenylate cyclase
