Difference between revisions of "Nap"

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=== Additional information===
 
=== Additional information===
 
 
  
  
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nap_592303_593205_-1 nap] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nap_592303_593205_-1 nap] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
'''Additional publications:'''  {{PubMed|22248594}}
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<pubmed> 12823818 24418394 22248594</pubmed>
<pubmed> 12823818 </pubmed>
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 08:55, 16 January 2014

  • Description: carboxylesterase NA

Gene name nap
Synonyms cesA
Essential no
Product carboxylesterase NA
Function lipid degradation
Gene expression levels in SubtiExpress: nap
MW, pI 33 kDa, 5.798
Gene length, protein length 900 bp, 300 aa
Immediate neighbours ydfJ, ydfK
Sequences Protein DNA DNA_with_flanks
Genetic context
Nap context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Nap expression.png
























Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05440

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: A carboxylic ester + H2O = an alcohol + a carboxylate (according to Swiss-Prot)
  • Protein family: AB hydrolase superfamily (according to Swiss-Prot)
  • Paralogous protein(s): YbfK

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Henriëtte J Rozeboom, Luis F Godinho, Marco Nardini, Wim J Quax, Bauke W Dijkstra
Crystal structures of two Bacillus carboxylesterases with different enantioselectivities.
Biochim Biophys Acta: 2014, 1844(3);567-75
[PubMed:24418394] [WorldCat.org] [DOI] (P p)

Luis F Godinho, Carlos R Reis, Henriëtte J Rozeboom, Frank J Dekker, Bauke W Dijkstra, Gerrit J Poelarends, Wim J Quax
Enhancement of the enantioselectivity of carboxylesterase A by structure-based mutagenesis.
J Biotechnol: 2012, 158(1-2);36-43
[PubMed:22248594] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)