NatK

• Description: two-component sensor kinase, regulation of the natA-natB operon
  
  

Gene name	natK
Synonyms	yccG
Essential	no
Product	two-component sensor kinase
Function	regulation of the natA-natB operon
Gene expression levels in SubtiExpress: natK
Interactions involving this protein in SubtInteract: NatK
MW, pI	34 kDa, 8.045
Gene length, protein length	954 bp, 318 aa
Immediate neighbours	yccF, natR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, membrane proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU02730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of NatR in response to sodium availability

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: three transmembrane segments, C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NatK-NatR

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P70954

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: natK (according to DBTBS)

• Expression browser: natK PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mitsuo Ogura, Kensuke Tsukahara, Kentaro Hayashi, Teruo Tanaka
The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion.
Microbiology (Reading): 2007, 153(Pt 3);667-675
[PubMed:17322186] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)