Difference between revisions of "AlkA"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[DNA repair/ recombination]]}}
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{{SubtiWiki category|[[DNA repair/ recombination]]}},
 +
{{SubtiWiki category|[[prophage 1]]}}
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alkA_202547_203458_-1 alkA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=alkA_202547_203458_-1 alkA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|8376346}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8376346}}
 
   
 
   
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 11:23, 11 September 2013

  • Description: DNA-3-methyladenine glycosylase

Gene name alkA
Synonyms
Essential no
Product DNA-3-methyladenine glycosylase
Function adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: alkA
MW, pI 35 kDa, 8.147
Gene length, protein length 909 bp, 303 aa
Immediate neighbours glmS, adaA
Sequences Protein DNA DNA_with_flanks
Genetic context
AlkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlkA expression.png















Categories containing this gene/protein

DNA repair/ recombination, prophage 1

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

  • Locus tag: BSU01800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)
  • Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: AdaA: positive regulation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)