Difference between revisions of "WalK"

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Revision as of 15:01, 16 May 2013

  • Description: two-component sensor kinase, controls cell wall metabolism, co-ordinates cell wall remodelling with cell division by controlling the transcription of genes for autolysins and their inhibitors

Gene name walK
Synonyms yycG
Essential yes PubMed
Product two-component sensor kinase (OmpR family)
Function control of cell wall metabolism
Gene expression levels in SubtiExpress: walK
Interactions involving this protein in SubtInteract: WalK
MW, pI 69 kDa, 4.775
Gene length, protein length 1833 bp, 611 aa
Immediate neighbours yycH, walR
Sequences Protein DNA DNA_with_flanks
Genetic context
YycG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WalK expression.png















Categories containing this gene/protein

cell wall/ other, protein modification, transcription factors and their control, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU40400

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of WalR
  • Protein family: two-component sensor kinase of the OmpR family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification: autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3SL2 (complex of the ATP binding histidine kinase domain with ATP) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during vegetative growth, repressed during stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Sarah Dubrac, Paola Bisicchia, Kevin M Devine, Tarek Msadek
A matter of life and death: cell wall homeostasis and the WalKR (YycGF) essential signal transduction pathway.
Mol Microbiol: 2008, 70(6);1307-22
[PubMed:19019149] [WorldCat.org] [DOI] (I p)

Sarah Dubrac, Tarek Msadek
Tearing down the wall: peptidoglycan metabolism and the WalK/WalR (YycG/YycF) essential two-component system.
Adv Exp Med Biol: 2008, 631;214-28
[PubMed:18792692] [WorldCat.org] [DOI] (P p)

Malcolm E Winkler, James A Hoch
Essentiality, bypass, and targeting of the YycFG (VicRK) two-component regulatory system in gram-positive bacteria.
J Bacteriol: 2008, 190(8);2645-8
[PubMed:18245295] [WorldCat.org] [DOI] (I p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)


Original Publications