Difference between revisions of "PrfB"

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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=prfB_3627139_3628240_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:prfB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35290]]
 
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Revision as of 14:41, 16 May 2013

  • Description: peptide chain release factor 2

Gene name prfB
Synonyms
Essential yes PubMed
Product peptide chain release factor 2
Function translation
Gene expression levels in SubtiExpress: prfB
Metabolic function and regulation of this protein in SubtiPathways:
Protein secretion
MW, pI 41 kDa, 4.85
Gene length, protein length 1099 bp, 366 aa
Immediate neighbours yvjA, secA
Sequences Protein DNA DNA_with_flanks
Genetic context
PrfB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrfB expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU35290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA
  • Protein family: prokaryotic/mitochondrial release factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References