Tgl

• Description: spore associated transglutaminase
  
  

Gene name	tgl
Synonyms	yuxF, yugV
Essential	no
Product	spore associated transglutaminase
Function	introduction of crosslinks in the spore coat protein GerQ
Gene expression levels in SubtiExpress: tgl
MW, pI	28 kDa, 6.687
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	mcpB, yuzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

• Locus tag: BSU31270

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH₃ (according to Swiss-Prot), temperature-dependent modification of the coat proteins such as GerQ (together with YabG) PubMed

• Protein family: bacillus TGase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P40746

• KEGG entry: [3]

• E.C. number: 2.3.2.13

Expression and regulation

• Operon: tgl PubMed

• Expression browser: tgl PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S122 ( tgl::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

Ritsuko Kuwana
[Protein modification system in dormant cells].
Yakugaku Zasshi: 2009, 129(10);1221-5
[PubMed:19797877] [WorldCat.org] [DOI] (P p)

Original Publications

Diana Plácido, Catarina G Fernandes, Anabela Isidro, Maria Arménia Carrondo, Adriano O Henriques, Margarida Archer
Auto-induction and purification of a Bacillus subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization.
Protein Expr Purif: 2008, 59(1);1-8
[PubMed:18249137] [WorldCat.org] [DOI] (I p)

Ritsuko Kuwana, Naoyuki Okuda, Hiromu Takamatsu, Kazuhito Watabe
Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores.
J Biochem: 2006, 139(5);887-901
[PubMed:16751597] [WorldCat.org] [DOI] (P p)

Rita Zilhão, Rachele Isticato, Lígia O Martins, Leif Steil, Uwe Völker, Ezio Ricca, Charles P Moran, Adriano O Henriques
Assembly and function of a spore coat-associated transglutaminase of Bacillus subtilis.
J Bacteriol: 2005, 187(22);7753-64
[PubMed:16267299] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Katsunori Kobayashi, Shun-Ichi Suzuki, Yuko Izawa, Kiyoshi Miwa, Shigeru Yamanaka
Transglutaminase in sporulating cells of Bacillus subtilis.
J Gen Appl Microbiol: 1998, 44(1);85-91
[PubMed:12501297] [WorldCat.org] [DOI] (I p)

S Suzuki, Y Izawa, K Kobayashi, Y Eto, S Yamanaka, K Kubota, K Yokozeki
Purification and characterization of novel transglutaminase from Bacillus subtilis spores.
Biosci Biotechnol Biochem: 2000, 64(11);2344-51
[PubMed:11193401] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Gregory Koningstein, Luísa Rodrigues, Maria de Sales Ramon, Helmut Hilbert, Andreas Düsterhöft, Thomas M Pohl, Thomas Weitzenegger
Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).
Microbiology (Reading): 1997, 143 ( Pt 8);2769-2774
[PubMed:9274030] [WorldCat.org] [DOI] (P p)