Difference between revisions of "Rph"
| Line 27: | Line 27: | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rph_2901154_2901891_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rph_expression.png|500px]] | + | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rph_2901154_2901891_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rph_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28370]] |
|- | |- | ||
|} | |} | ||
Revision as of 14:12, 16 May 2013
- Description: RNase PH, 3'-5' exoribonuclease
| Gene name | rph |
| Synonyms | |
| Essential | no |
| Product | RNase PH (EC 2.7.7.56) |
| Function | 3'-5' exoribonuclease |
| Gene expression levels in SubtiExpress: rph | |
| MW, pI | 26 kDa, 4.888 |
| Gene length, protein length | 735 bp, 245 aa |
| Immediate neighbours | ysnA, gerM |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28370
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: RNase PH family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1OYS
- UniProt: P28619
- KEGG entry: [2]
- E.C. number: 2.7.7.56
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Yulia Redko, Ciarán Condon
Maturation of 23S rRNA in Bacillus subtilis in the absence of Mini-III.
J Bacteriol: 2010, 192(1);356-9
[PubMed:19880604]
[WorldCat.org]
[DOI]
(I p)
Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666]
[WorldCat.org]
[DOI]
(I p)
Tingyi Wen, Irina A Oussenko, Olivier Pellegrini, David H Bechhofer, Ciarán Condon
Ribonuclease PH plays a major role in the exonucleolytic maturation of CCA-containing tRNA precursors in Bacillus subtilis.
Nucleic Acids Res: 2005, 33(11);3636-43
[PubMed:15983136]
[WorldCat.org]
[DOI]
(I e)
Irina A Oussenko, Teppei Abe, Hiromi Ujiie, Akira Muto, David H Bechhofer
Participation of 3'-to-5' exoribonucleases in the turnover of Bacillus subtilis mRNA.
J Bacteriol: 2005, 187(8);2758-67
[PubMed:15805522]
[WorldCat.org]
[DOI]
(P p)
Lene S Harlow, Anders Kadziola, Kaj Frank Jensen, Sine Larsen
Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding.
Protein Sci: 2004, 13(3);668-77
[PubMed:14767080]
[WorldCat.org]
[DOI]
(P p)
M G Craven, D J Henner, D Alessi, A T Schauer, K A Ost, M P Deutscher, D I Friedman
Identification of the rph (RNase PH) gene of Bacillus subtilis: evidence for suppression of cold-sensitive mutations in Escherichia coli.
J Bacteriol: 1992, 174(14);4727-35
[PubMed:1624460]
[WorldCat.org]
[DOI]
(P p)
