Difference between revisions of "GndA"
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Revision as of 13:50, 16 May 2013
- Description: NADP-dependent phosphogluconate dehydrogenase
| Gene name | gndA |
| Synonyms | yqjI |
| Essential | no |
| Product | NADP-dependent phosphogluconate dehydrogenase |
| Function | pentose phosphate pathway |
| Gene expression levels in SubtiExpress: gndA | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 51 kDa, 5.076 |
| Gene length, protein length | 1407 bp, 469 aa |
| Immediate neighbours | zwf, polY1 |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23860
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH (according to Swiss-Prot)
- Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Reversible reaction PubMed
- Domains:
- Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P80859
- KEGG entry: [3]
- E.C. number: 1.1.1.44
Additional information
It contains a cysteine on the active site. The enzyme is a dimer. PubMed
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1514 (gndA::kan), available in Stülke lab
- Expression vector:
- pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
- pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Stülke lab)
- GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
- FLAG-tag construct:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Scott Cameron, Viviane P Martini, Jorge Iulek, William N Hunter
Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 5);450-4
[PubMed:19407374]
[WorldCat.org]
[DOI]
(I p)
Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307]
[WorldCat.org]
[DOI]
(P p)
