Difference between revisions of "Cca"
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Revision as of 13:44, 16 May 2013
- Description: tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3' end
| Gene name | cca |
| Synonyms | papS, ypjI |
| Essential | yes PubMed |
| Product | tRNA nucleotidyltransferase |
| Function | tRNA modification |
| Gene expression levels in SubtiExpress: cca | |
| MW, pI | 45 kDa, 8.041 |
| Gene length, protein length | 1191 bp, 397 aa |
| Immediate neighbours | birA, bshA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22450
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)
- Protein family: the protein is similar to the E. coli poly(A) polymerase
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1MIV (Geobacillus stearothermophilus 44% identity)
- UniProt: P42977
- KEGG entry: [2]
- E.C. number: 2.7.7.25
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Heike Betat, Christiane Rammelt, Mario Mörl
tRNA nucleotidyltransferases: ancient catalysts with an unusual mechanism of polymerization.
Cell Mol Life Sci: 2010, 67(9);1447-63
[PubMed:20155482]
[WorldCat.org]
[DOI]
(I p)
Stefan Vörtler, Mario Mörl
tRNA-nucleotidyltransferases: highly unusual RNA polymerases with vital functions.
FEBS Lett: 2010, 584(2);297-302
[PubMed:19883645]
[WorldCat.org]
[DOI]
(I p)
Anne Neuenfeldt, Andrea Just, Heike Betat, Mario Mörl
Evolution of tRNA nucleotidyltransferases: a small deletion generated CC-adding enzymes.
Proc Natl Acad Sci U S A: 2008, 105(23);7953-8
[PubMed:18523015]
[WorldCat.org]
[DOI]
(I p)
Yong Xiong, Thomas A Steitz
A story with a good ending: tRNA 3'-end maturation by CCA-adding enzymes.
Curr Opin Struct Biol: 2006, 16(1);12-7
[PubMed:16364630]
[WorldCat.org]
[DOI]
(P p)
Original publications
Operon and expression
