Difference between revisions of "RbgA"
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Revision as of 13:20, 16 May 2013
- Description: assembly of the 50S subunit of the ribosome
| Gene name | rbgA |
| Synonyms | ylqF |
| Essential | yes PubMed |
| Product | GTPase |
| Function | ribosome assembly |
| Gene expression levels in SubtiExpress: rbgA | |
| MW, pI | 31 kDa, 9.528 |
| Gene length, protein length | 846 bp, 282 aa |
| Immediate neighbours | rplS, rnhB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
translation, essential genes, GTP-binding proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU16050
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
- RNA-binding domain at the C-terminus PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1PUJ
- UniProt: O31743
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Naotake Ogasawara, Nara, Japan
Your additional remarks
References
Reviews
Original publications
Additional publications: PubMed
Megha Gulati, Nikhil Jain, Baskaran Anand, Balaji Prakash, Robert A Britton
Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation.
Nucleic Acids Res: 2013, 41(5);3217-27
[PubMed:23325847]
[WorldCat.org]
[DOI]
(I p)
Laura Schaefer, William C Uicker, Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Jean-Michel Jault, Robert A Britton
Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis.
J Bacteriol: 2006, 188(23);8252-8
[PubMed:16997968]
[WorldCat.org]
[DOI]
(P p)
William C Uicker, Laura Schaefer, Robert A Britton
The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis.
Mol Microbiol: 2006, 59(2);528-40
[PubMed:16390447]
[WorldCat.org]
[DOI]
(P p)
Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945]
[WorldCat.org]
[DOI]
(P p)
