Difference between revisions of "PGP574"
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pGP574 | pGP574 | ||
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| + | vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein | ||
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| + | host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
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| + | constructed in the [[Stülke]] lab | ||
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| + | in E. coli: ampicillin resistance | ||
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| + | based on pET3C | ||
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| + | Schirmer et al. (1997) J. Bacteriol. '''179:''' 1329-1336. [http://www.ncbi.nlm.nih.gov/sites/entrez/9023219 PubMed] | ||
Revision as of 10:31, 15 January 2009
pGP574
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed
