RsfA

• Description: probable regulator of transcription of SigF-dependent genes
  
  

Gene name	rsfA
Synonyms	ywfN, ipa-92r
Essential	no
Product	regulator of SigF activity
Function	control of expression of SigF-dependent genes
Gene expression levels in SubtiExpress: rsfA
MW, pI	29 kDa, 5.13
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	ywzC, ywfM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

RsfA regulon, SigF regulon, SigG regulon

The RsfA regulon:

• katX, rsfA

The gene

Basic information

• Locus tag: BSU37620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • forespore (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39650

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rsfA PubMed

• Expression browser: rsfA PubMed

• Sigma factor: SigF PubMed, SigG PubMed

• Regulation:
  • expressed during sporulation in the forespore (SigF) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S132 ( rsfA::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis.
J Bacteriol: 2000, 182(2);418-24
[PubMed:10629188] [WorldCat.org] [DOI] (P p)

P Glaser, F Kunst, M Arnaud, M P Coudart, W Gonzales, M F Hullo, M Ionescu, B Lubochinsky, L Marcelino, I Moszer
Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees.
Mol Microbiol: 1993, 10(2);371-84
[PubMed:7934828] [WorldCat.org] (P p)