GlyA

• Description: serine hydroxymethyltransferase
  
  

Gene name	glyA
Synonyms	glyC, ipc-34d
Essential	yes PubMed
Product	serine hydroxymethyltransferase
Function	biosynthesis of glycine
Gene expression levels in SubtiExpress: glyA
Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser
MW, pI	45 kDa, 5.475
Gene length, protein length	1245 bp, 415 aa
Immediate neighbours	upp, ywlG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes, phosphoproteins

This gene is a member of the following regulons

PurR regulon, T-box

The gene

Basic information

• Locus tag: BSU36900

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + glycine + H₂O = tetrahydrofolate + L-serine (according to Swiss-Prot)

• Protein family: SHMT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2VGU (complex with L-serine, Geobacillus stearothermophilus), 2VI8 (Geobacillus stearothermophilus)

• UniProt: P39148

• KEGG entry: [3]

• E.C. number: 2.1.2.1

Expression and regulation

• Operon: glyA (according to DBTBS)

• Expression browser: glyA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • induced by glycine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: