LytD

• Description: glucosaminidase
  
  

Gene name	lytD
Synonyms	cwlG
Essential	no
Product	glucosaminidase
Function	major autolysin, cell separation
Gene expression levels in SubtiExpress: lytD
MW, pI	95 kDa, 9.935
Gene length, protein length	2640 bp, 880 aa
Immediate neighbours	tagC, pmi
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU35780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)₂)Asn-structure (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 73 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P39848

• KEGG entry: [3]

• E.C. number: 3.2.1.96

Expression and regulation

• Operon: lytD PubMed

• Expression browser: lytD PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A788 ( lytD::tet), PubMed, available at BGSC
  • 1A792 ( lytD::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: