Difference between revisions of "PAC6"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
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| − | vector for | + | pAC6 |
| − | constructed in the | + | |
| + | vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
| + | |||
| + | does not replicate in ''B. subtilis'', integrates into the ''amyE'' site | ||
| + | |||
| + | constructed in the Rapoport lab | ||
| + | |||
in E. coli: ampicillin resistance | in E. coli: ampicillin resistance | ||
| − | in B. subtilis: | + | |
| − | + | in B. subtilis: chloramphenocol resistance | |
| − | + | ||
| + | similar vectors: [[pAC5]], [[pAC7]] | ||
| + | |||
| + | Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/1619665 PubMed] | ||
Revision as of 10:04, 15 January 2009
pAC6
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenocol resistance
Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. PubMed
