Difference between revisions of "MutL"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutS]]'', ''[[ymzD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mutS]]'', ''[[ymzD]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17050 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17050 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17050 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:mutL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mutL_context.gif]]

Revision as of 10:17, 14 May 2013

  • Description: DNA mismatch repair

Gene name mutL
Synonyms
Essential no
Product DNA mismatch repair
Function DNA repair
Gene expression levels in SubtiExpress: mutL
Interactions involving this protein in SubtInteract: MutL
MW, pI 70 kDa, 5.583
Gene length, protein length 1881 bp, 627 aa
Immediate neighbours mutS, ymzD
Sequences Protein DNA DNA_with_flanks
Genetic context
MutL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MutL expression.png
























Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU17050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: DNA mismatch repair mutL/hexB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant: GP1190 (del mutSL::aphA3) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Nicholas J Bolz, Justin S Lenhart, Steven C Weindorf, Lyle A Simmons
Residues in the N-terminal domain of MutL required for mismatch repair in Bacillus subtilis.
J Bacteriol: 2012, 194(19);5361-7
[PubMed:22843852] [WorldCat.org] [DOI] (I p)

Monica C Pillon, Jessica J Lorenowicz, Michael Uckelmann, Andrew D Klocko, Ryan R Mitchell, Yu Seon Chung, Paul Modrich, Graham C Walker, Lyle A Simmons, Peter Friedhoff, Alba Guarné
Structure of the endonuclease domain of MutL: unlicensed to cut.
Mol Cell: 2010, 39(1);145-51
[PubMed:20603082] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mario Pedraza-Reyes, Ronald E Yasbin
Contribution of the mismatch DNA repair system to the generation of stationary-phase-induced mutants of Bacillus subtilis.
J Bacteriol: 2004, 186(19);6485-91
[PubMed:15375129] [WorldCat.org] [DOI] (P p)

F Ginetti, M Perego, A M Albertini, A Galizzi
Bacillus subtilis mutS mutL operon: identification, nucleotide sequence and mutagenesis.
Microbiology (Reading): 1996, 142 ( Pt 8);2021-9
[PubMed:8760914] [WorldCat.org] [DOI] (P p)