Difference between revisions of "YkuI"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuH]]'', ''[[fsrA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuH]]'', ''[[fsrA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14090 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14090 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14090 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14090 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ykuI_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykuI_context.gif]]

Revision as of 10:04, 14 May 2013

  • Description: potential c-di-GMP degrading phosphodiesterase

Gene name ykuI
Synonyms
Essential no
Product potential c-di-GMP degrading phosphodiesterase
Function unknown
Gene expression levels in SubtiExpress: ykuI
MW, pI 47 kDa, 4.462
Gene length, protein length 1221 bp, 407 aa
Immediate neighbours ykuH, fsrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkuI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkuI expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14090

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251] [WorldCat.org] [DOI] (P p)