Difference between revisions of "HemH"
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemE]]'', ''[[hemY]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hemE]]'', ''[[hemY]]'' | ||
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| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10130 | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10130 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10130 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10130 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:hemH_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:hemH_context.gif]] | ||
Revision as of 09:50, 14 May 2013
- Description: ferrochelatase
| Gene name | hemH |
| Synonyms | hemF |
| Essential | no |
| Product | ferrochelatase |
| Function | heme biosynthesis |
| Gene expression levels in SubtiExpress: hemH | |
| MW, pI | 35 kDa, 4.617 |
| Gene length, protein length | 930 bp, 310 aa |
| Immediate neighbours | hemE, hemY |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU10130
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: insertion of Fe(2+) into protoporphyrin IX
- Protein family: ferrochelatase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P32396
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yaxue Wang, Yong Shen
Is it possible for Fe2+ to approach protoporphyrin IX from the side of Tyr-13 in Bacillus subtilis ferrochelatase? An answer from QM/MM study.
J Mol Model: 2013, 19(2);963-71
[PubMed:23097001]
[WorldCat.org]
[DOI]
(I p)
Mattias D Hansson, Tobias Karlberg, Christopher A G Söderberg, Sreekanth Rajan, Martin J Warren, Salam Al-Karadaghi, Stephen E J Rigby, Mats Hansson
Bacterial ferrochelatase turns human: Tyr13 determines the apparent metal specificity of Bacillus subtilis ferrochelatase.
J Biol Inorg Chem: 2011, 16(2);235-42
[PubMed:21052751]
[WorldCat.org]
[DOI]
(I p)
Mattias D Hansson, Mats Lindstam, Mats Hansson
Crosstalk between metal ions in Bacillus subtilis ferrochelatase.
J Biol Inorg Chem: 2006, 11(3);325-33
[PubMed:16453119]
[WorldCat.org]
[DOI]
(P p)
S Al-Karadaghi, M Hansson, S Nikonov, B Jönsson, L Hederstedt
Crystal structure of ferrochelatase: the terminal enzyme in heme biosynthesis.
Structure: 1997, 5(11);1501-10
[PubMed:9384565]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Purification and characterisation of a water-soluble ferrochelatase from Bacillus subtilis.
Eur J Biochem: 1994, 220(1);201-8
[PubMed:8119288]
[WorldCat.org]
[DOI]
(P p)
M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957]
[WorldCat.org]
[DOI]
(P p)
