YesW

• Description: rhamnogalacturonan lyase, generates oligosaccharides
  
  

Gene name	yesW
Synonyms
Essential	no
Product	rhamnogalacturonan lyase, generates oligosaccharides
Function	utilization of rhamnogalacturonan
Gene expression levels in SubtiExpress: yesW
MW, pI	67 kDa, 5.966
Gene length, protein length	1860 bp, 620 aa
Immediate neighbours	yesV, yesX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

YesS regulon

The gene

Basic information

• Locus tag: BSU07050

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: FG-GAP repeat (according to Swiss-Prot)

• Paralogous protein(s): YesX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• Structure: 2Z8R

• UniProt: O31526

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yesS-yesT-yesU-yesV-yesW-yesX-yesY-yesZ PubMed

• Expression browser: yesW PubMed

• Sigma factor:

• Regulation: induced by pectin (YesS) PubMed

• Regulatory mechanism: YesS: transcriptional activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sandrine Poncet, Maryline Soret, Peggy Mervelet, Josef Deutscher, Philippe Noirot
Transcriptional activator YesS is stimulated by histidine-phosphorylated HPr of the Bacillus subtilis phosphotransferase system.
J Biol Chem: 2009, 284(41);28188-28197
[PubMed:19651770] [WorldCat.org] [DOI] (I p)

Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases.
J Biol Chem: 2009, 284(15);10181-9
[PubMed:19193638] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Yukie Maruyama, Akiko Kawamata, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller.
J Biol Chem: 2007, 282(51);37134-45
[PubMed:17947240] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)

Akihito Ochiai, Masayuki Yamasaki, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 5);438-40
[PubMed:16682770] [WorldCat.org] [DOI] (I p)