Difference between revisions of "YabT"
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of DNA integrity during spore development | |style="background:#ABCDEF;" align="center"|'''Function''' || control of DNA integrity during spore development | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00660 yabT] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabT YabT] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/YabT YabT] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabS]]'', ''[[tilS]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabS]]'', ''[[tilS]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00660 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00660 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00660 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yabT_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yabT_context.gif]] | ||
Revision as of 11:56, 13 May 2013
- Description: Ser/Thr kinase, controls DNA integrity during spore development
| Gene name | yabT |
| Synonyms | |
| Essential | no |
| Product | Ser/Thr kinase |
| Function | control of DNA integrity during spore development |
| Gene expression levels in SubtiExpress: yabT | |
| Interactions involving this protein in SubtInteract: YabT | |
| MW, pI | 37 kDa, 9.943 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yabS, tilS |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
protein modification, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00660
Phenotypes of a mutant
- increased sensitivity to DNA damage during spore development PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: protein kinase domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- autophosphorylation is stimulated by non-specific binding to DNA PubMed
- Localization:
- colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed
Database entries
- Structure:
- UniProt: P37562
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP577 (erm), available in Jörg Stülke's lab
- Expression vector:
- purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
- purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
- purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
- lacZ fusion:
- translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
