Difference between revisions of "SplB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA against photodamage
 
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA against photodamage
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13930 splB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13930 splB]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 8.524   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 8.524   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[splA]]'', ''[[ykwB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[splA]]'', ''[[ykwB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13266&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13930 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:splB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:splB_context.gif]]

Revision as of 12:41, 13 May 2013

  • Description: spore photoproduct lyase

Gene name splB
Synonyms spl
Essential no
Product spore photoproduct lyase
Function protection of spore DNA against photodamage
Gene expression levels in SubtiExpress: splB
MW, pI 39 kDa, 8.524
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours splA, ykwB
Sequences Protein DNA Advanced_DNA
Genetic context
SplB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SplB expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU13930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
    • 5-thyminyl-5,6-dihydrothymine (in DNA) = 2 thymine residues (in DNA) (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
    • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Linlin Yang, Renae S Nelson, Alhosna Benjdia, Gengjie Lin, Joshua Telser, Stefan Stoll, Ilme Schlichting, Lei Li
A radical transfer pathway in spore photoproduct lyase.
Biochemistry: 2013, 52(18);3041-50
[PubMed:23607538] [WorldCat.org] [DOI] (I p)

Lei Li
Mechanistic studies of the radical SAM enzyme spore photoproduct lyase (SPL).
Biochim Biophys Acta: 2012, 1824(11);1264-77
[PubMed:22197590] [WorldCat.org] [DOI] (P p)

Linlin Yang, Gengjie Lin, Degang Liu, Karl J Dria, Joshua Telser, Lei Li
Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.
J Am Chem Soc: 2011, 133(27);10434-47
[PubMed:21671623] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

M Pedraza-Reyes, F Gutiérrez-Corona, W L Nicholson
Temporal regulation and forespore-specific expression of the spore photoproduct lyase gene by sigma-G RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1994, 176(13);3983-91
[PubMed:8021181] [WorldCat.org] [DOI] (P p)