Difference between revisions of "SleB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || degration of the spore cortex, germination | |style="background:#ABCDEF;" align="center"|'''Function''' || degration of the spore cortex, germination | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22930 sleB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.506 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 9.506 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypeB]]'', ''[[prsW]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ypeB]]'', ''[[prsW]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22930 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:sleB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:sleB_context.gif]] | ||
Revision as of 13:10, 13 May 2013
- Description: spore cortex-lytic enzyme
| Gene name | sleB |
| Synonyms | ypeA |
| Essential | no |
| Product | spore cortex-lytic enzyme |
| Function | degration of the spore cortex, germination |
| Gene expression levels in SubtiExpress: sleB | |
| MW, pI | 33 kDa, 9.506 |
| Gene length, protein length | 915 bp, 305 aa |
| Immediate neighbours | ypeB, prsW |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
germination, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22930
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sleB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains a signal peptide PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- outer surface of the inner spore membrane PubMed
Database entries
- Structure:
- UniProt: P50739
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708]
[WorldCat.org]
[DOI]
(I p)
George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419]
[WorldCat.org]
[DOI]
(I p)
Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722]
[WorldCat.org]
[DOI]
(I p)
Atsushi Masayama, Hideyuki Fukuoka, Shiro Kato, Tohru Yoshimura, Michie Moriyama, Ryuichi Moriyama
Subcellular localization of a germiantion-specific cortex-lytic enzyme, SleB, of Bacilli during sporulation.
Genes Genet Syst: 2006, 81(3);163-9
[PubMed:16905870]
[WorldCat.org]
[DOI]
(P p)
Barbara Setlow, Swaroopa Atluri, Ryan Kitchel, Kasia Koziol-Dube, Peter Setlow
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.
J Bacteriol: 2006, 188(11);3740-7
[PubMed:16707666]
[WorldCat.org]
[DOI]
(P p)
R Moriyama, H Fukuoka, S Miyata, S Kudoh, A Hattori, S Kozuka, Y Yasuda, K Tochikubo, S Makino
Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.
J Bacteriol: 1999, 181(8);2373-8
[PubMed:10197998]
[WorldCat.org]
[DOI]
(P p)
