Difference between revisions of "PcrA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || plasmid rolling-circle replication, conjugative transfer of ICEBs1
 
|style="background:#ABCDEF;" align="center"|'''Function''' || plasmid rolling-circle replication, conjugative transfer of ICEBs1
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06610 pcrA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06610 pcrA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PcrA PcrA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PcrA PcrA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrB]]'', ''[[ligA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrB]]'', ''[[ligA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12481&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06610 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:pcrA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pcrA_context.gif]]

Revision as of 12:17, 13 May 2013

  • Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

Gene name pcrA
Synonyms yerF
Essential yes PubMed
Product ATP-dependent DNA helicase
Function plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI 83 kDa, 5.767
Gene length, protein length 2217 bp, 739 aa
Immediate neighbours pcrB, ligA
Sequences Protein DNA Advanced_DNA
Genetic context
PcrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PcrA expression.png















Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
    • dismantles RecA filaments PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s): YjcD

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):

Database entries

  • Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications

Additional publications: PubMed