Difference between revisions of "RipX"

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|style="background:#ABCDEF;" align="center"|'''Function''' || chromosome partitioning
 
|style="background:#ABCDEF;" align="center"|'''Function''' || chromosome partitioning
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23510 ripX]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23510 ripX]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 10   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 10   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[drm]]'', ''[[yqzK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[drm]]'', ''[[yqzK]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14283&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23510 Advanced_DNA]
 
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Revision as of 13:12, 13 May 2013

  • Description: site-specific integrase/recombinase

Gene name ripX
Synonyms ykqM
Essential no
Product site-specific integrase/recombinase
Function chromosome partitioning
Gene expression levels in SubtiExpress: ripX
MW, pI 33 kDa, 10
Gene length, protein length 888 bp, 296 aa
Immediate neighbours drm, yqzK
Sequences Protein DNA Advanced_DNA
Genetic context
RipX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RipX expression.png


























Categories containing this gene/protein

DNA condensation/ segregation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23510

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)
  • Protein family: XerD subfamily (according to Swiss-Prot)
  • Paralogous protein(s): CodV

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • cytoplasm (according to Swiss-Prot)
    • assembles on the chromosome at the dif site throughout the cell cycle PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Alexandra E Bloor, Rocky M Cranenburgh
An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes.
Appl Environ Microbiol: 2006, 72(4);2520-5
[PubMed:16597952] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, G W Blakely
Identification and characterization of the dif Site from Bacillus subtilis.
J Bacteriol: 2001, 183(3);1058-68
[PubMed:11208805] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, D J Sherratt, G Blakely
The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning.
J Bacteriol: 1999, 181(19);6053-62
[PubMed:10498718] [WorldCat.org] [DOI] (P p)