Difference between revisions of "CheD"
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| − | * '''Description:''' | + | * '''Description:''' chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by [[CheR]], enhances phosphatase activity of [[CheC]], required for full [[McpC]] activity <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| + | ** binds chemoreceptors and increases their ability to activate the kinase activity of [[CheA]] {{PubMed|23226535}} | ||
** Protein L-glutamine + H<sub>2</sub>O = protein L-glutamate + NH<sub>3</sub> (according to Swiss-Prot) | ** Protein L-glutamine + H<sub>2</sub>O = protein L-glutamate + NH<sub>3</sub> (according to Swiss-Prot) | ||
** deaminates Gln-586, Gln-593, and Gln594 in [[McpA]] {{PubMed|22931217}} | ** deaminates Gln-586, Gln-593, and Gln594 in [[McpA]] {{PubMed|22931217}} | ||
| Line 91: | Line 92: | ||
* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| − | ** [[CheC]]-[[CheD]] {{PubMed|17908686}} | + | ** [[CheC]]-[[CheD]] {{PubMed|17908686}}, this interaction is enhanced by [[CheY]]-P {{PubMed|23226535}} |
** [[CheD]]-[[McpA]], deamination of Gln-586, Gln-593 and Gln-594 in [[McpA]] {{PubMed|22931217}} | ** [[CheD]]-[[McpA]], deamination of Gln-586, Gln-593 and Gln-594 in [[McpA]] {{PubMed|22931217}} | ||
** [[CheD]]-[[McpC]], deamination of Gln-609 in [[McpC]] {{PubMed|22931217}} | ** [[CheD]]-[[McpC]], deamination of Gln-609 in [[McpC]] {{PubMed|22931217}} | ||
| Line 155: | Line 156: | ||
<pubmed>,18774298,,18990184</pubmed> | <pubmed>,18774298,,18990184</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647, 9657996,8157612,15175317,16469702 17850253 21515776 22931217</pubmed> | + | <pubmed>7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647, 9657996,8157612,15175317,16469702 17850253 21515776 22931217 23226535</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:54, 3 January 2013
- Description: chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
| Gene name | cheD |
| Synonyms | ylxK |
| Essential | no |
| Product | protein deaminase, CheC activity modulator |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: cheD | |
| Interactions involving this protein in SubtInteract: CheD | |
| MW, pI | 17 kDa, 9.347 |
| Gene length, protein length | 498 bp, 166 aa |
| Immediate neighbours | cheC, sigD |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
protein modification, motility and chemotaxis
This gene is a member of the following regulons
CodY regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cheD family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P40404
- KEGG entry: [3]
- E.C. number: 3.5.1.44
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
- in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell PubMed
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
