YjbH

• Description: adaptor protein for ClpX-ClpP-catalyzed Spx degradation, confers resistance against nitrosating agents
  
  

Gene name	yjbH
Synonyms
Essential	no
Product	adaptor protein
Function	stimulation of Spx degradation
Gene expression levels in SubtiExpress: yjbH
Interactions involving this protein in SubtInteract: YjbH
MW, pI	31 kDa, 5.206
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	yizD, yjbI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteolysis, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

The gene

Basic information

• Locus tag: BSU11550

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: adaptor protein for ClpX-ClpP-catalyzed Spx degradation PubMed

• Protein family: UPF0413 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):contains Zn atoms (coordinated by the N-terminal His-rich region) PubMed

• Effectors of protein activity:
  • Zn atom is released upon treatment with strong oxidants PubMed
  • interaction with YirB inhibits the formation of a complex with Spx PubMed

• Interactions:
  • YjbH-Spx PubMed
  • YirB-YjbH PubMed

• Localization: cytosolic protein

Database entries

• Structure:

• UniProt: O31606

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yjbI-yjbH PubMed

• Expression browser: yjbH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • A ncRNA is predicted between yizD and yjbH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Zuber, Oregon Health and Science University, USA Homepage

Claes von Wachenfeldt, Lund University, Sweden Homepage

References

Reviews: PubMed

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Saurabh K Garg, Sushma Kommineni, Luke Henslee, Ying Zhang, Peter Zuber
The YjbH protein of Bacillus subtilis enhances ClpXP-catalyzed proteolysis of Spx.
J Bacteriol: 2009, 191(4);1268-77
[PubMed:19074380] [WorldCat.org] [DOI] (I p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
YjbH is a novel negative effector of the disulphide stress regulator, Spx, in Bacillus subtilis.
Mol Microbiol: 2007, 66(3);669-84
[PubMed:17908206] [WorldCat.org] [DOI] (P p)

Annika Rogstam, Jonas T Larsson, Peter Kjelgaard, Claes von Wachenfeldt
Mechanisms of adaptation to nitrosative stress in Bacillus subtilis.
J Bacteriol: 2007, 189(8);3063-71
[PubMed:17293416] [WorldCat.org] [DOI] (P p)