TsaB

• Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA
  
  

Gene name	tsaB
Synonyms	ydiC
Essential	yes PubMed
Product	tRNA modification enzyme
Function	tRNA modification
Gene expression levels in SubtiExpress: tsaB
Interactions involving this protein in SubtInteract: TsaB
MW, pI	25 kDa, 5.762
Gene length, protein length	687 bp, 229 aa
Immediate neighbours	tsaE, ydiD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

essential genes, translation

The gene

Basic information

• Locus tag: BSU05920

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TsaB-TsaD PubMed
  • TsaB-TsaE PubMed

• Localization:

Database entries

• Structure:

• UniProt: O05516

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: thiL-tsaE-tsaB-ydiD-tsaD PubMed

• Expression browser: tsaB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: